Fracture characterisation under mode I loading of Eucalyptus globulus Labill. bonded joints with one-component polyurethane adhesive (1C-PUR) is addressed in this work. The objective is to estimate the cohesive law representative of the fracture behaviour of these joints. A direct and two inverse procedures were employed to determine the softening laws. The direct method is based on local measurement of crack tip displacements using digital image correlation in the course of Double Cantilever Beam (DCB) tests, while the inverse methods rely on load–displacement data and on load-crack tip opening displacement relations by finite element modelling. It was verified that consistent results can be obtained from the three methodologies leading to the conclusion that the classical inverse procedure is the most appealing one owing to its simplicity.