In this work, the identifiability of orthotropic stiffness components of clear wood from a single off-axis compression test was investigated. The material characterisation strategy was based on anisotropic elasticity. Moreover, it requires 3D full-field measurements which can be provided by a suitable optical technique such as stereovision. Finite element analyses of the mechanical test method were carried out in order to optimise the test configuration. Results were discussed in view of the identifiability of all active stiffness components from only one test configuration.